All chromatographic separations, which include HPLC function under the similar basic principle; every compound interacts with other chemical species inside of a attribute manner.
Where by N is the quantity of theoretical plates, L will be the length of your column, and H is the height reminiscent
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Compound separation. Actual physical separation of your compounds comes about over the column stationary section. Immediately after elution with the column, the divided sample elements travel to the detector.
Each individual analyte while in the sample interacts a bit differently Using the adsorb
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